Here are the maps for the AdZ vectors. Plasmid maps are in genbank format - this can be imported by most molecular biology programs. It's a text format which includes both sequence & annotations. They're also here as plain text (Fasta format) and a pdf which lists the positions of some of their features. Note that the vector numbers are different to those in the original publication - the original work used vectors in which the genome was derived from the AdEasy vectors. The new vectors contain a newly derived & fully sequenced Ad5 genome, but the expression cassettes etc are identical to those in the original paper. In addition to these E1/E3 deleted vectors we also have versions that are E3 intact, or fully intact. The maps for those are at the bottom.
pAdZ5-CV5 - this is the entire sequence, including all the Ad backbone.
Genbank Format Text (Fasta) Format Pdf
The following maps are all just the expression cassettes - the remaining sequence is identical to pAdZ5-CV5 above.
pAdZ5-CV5 - for adding C terminal V5 tags
Genbank Format Text (Fasta) Format Pdf
pAdZ5-NV5 for adding N terminal V5 tags
Genbank Format Text (Fasta) Format Pdf
pAdZ5-NGFP for adding N terminal GFP tags
Genbank Format Text (Fasta) Format Pdf
pAdZ5-CGFP for adding C terminal GFP tags
Genbank Format Text (Fasta) Format Pdf
pAdZ5-CmCherry for adding C terminal mCherry tags
Genbank Format Text (Fasta) Format Pdf
pAdZ5-mIR155 for cloning shRNAs
Genbank Format Text (Fasta) Format Pdf
pAdZ5-CStrep2 for adding C terminal Strep-2 or strep-3 tags
Genbank Format Text (Fasta) Format Pdf
pAdZ5-CV5-NT for adding C terminal V5 tags, promoter lacks tet operators
Genbank Format Text (Fasta) Format Pdf
pAdZ5-CGFP-NT for adding C terminal GFP tags, promoter lacks tet operators
Genbank Format Text (Fasta) Format Pdf
pAdZ5-Ctrl is empty vector control, containing just a V5 tag inbetween the promoter & polyA
Genbank Format Text (Fasta) Format Pdf
pAdZ5 is the entire Ad5 genome, in a non-self-excising vector. If you wish to recover virus from this vector, it must first be linearised by PacI or SwaI digestion. If you want to make a self-excising wildtype Ad vector, then simply replace the expression cassette in pAdZ5-CV5-E3+ (below) with the E1 region from pAdZ5 in a single recombineering step.
Genbank Format Text (Fasta) Format
pAdZ5-CV5-E3+ is the same as pAdZ5-CV5, but is intact in the E3 region
Genbank Format Text (Fasta) Format