LOCUS       pAL1256\for\webs        5727 bp    DNA     linear       12-SEP-2008
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|493234425|
COMMENT     VNTDBDATE|493234572|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAL1256 for website|
COMMENT     VNTAUTHORNAME|Rich Stanton|
FEATURES             Location/Qualifiers
     promoter        1295..1393
                     /invitrogen="2330000"
                     /vntifkey="30"
                     /label=bla\promoter
     CDS             1394..2254
                     /invitrogen="1020000"
                     /vntifkey="4"
                     /label=Amp(R)
     primer          1187..1208
                     /vntifkey="27"
                     /label=Primer\-L
     CDS             3586..5007
                     /vntifkey="4"
                     /label=sacB
     primer          complement(5383..5403)
                     /vntifkey="27"
                     /label=Primer-R
     CDS             complement(2561..2914)
                     /vntifkey="4"
                     /label=LacZa
     CDS             443..1159
                     /vntifkey="4"
                     /label=eGFP
     misc_feature    308..326
                     /vntifkey="21"
                     /label=tet\operator
     misc_feature    329..347
                     /vntifkey="21"
                     /label=tet\Operator
     promoter        21..430
                     /vntifkey="29"
                     /label=CMV\Promotor
     polyA_signal    5443..5721
                     /vntifkey="25"
                     /label=CMV\IE\PolyA
     misc_feature    1160..1186
                     /vntifkey="21"
                     /label=Linker
BASE COUNT     1621 a      1390 c      1321 g      1395 t 
ORIGIN
        1 aagcttgcat gcctgcaggt ctggcattat gcccagtaca tgaccttatg ggactttcct 
       61 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag 
      121 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt 
      181 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac 
      241 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc 
      301 agagctctcc ctatcagtga tagagatctc cctatcagtg atagagagtt tagtgaaccg 
      361 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg 
      421 atccagcctg gatcccgcca ccatggtgag caagggcgag gagctgttca ccggggtggt 
      481 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga 
      541 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa 
      601 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag 
      661 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta 
      721 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt 
      781 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga 
      841 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat 
      901 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga 
      961 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc 
     1021 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa 
     1081 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg 
     1141 catggacgag ctgtacaagg gtagcgctgg atcagcaggg tccgcgcctg tgacggaaga 
     1201 tcacttcgat tgtactgaga gtcgaccata ggggatcggg agatctcccg atccgtcgac 
     1261 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat 
     1321 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg 
     1381 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc 
     1441 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga 
     1501 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga 
     1561 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg 
     1621 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc 
     1681 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac 
     1741 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact 
     1801 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca 
     1861 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg 
     1921 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact 
     1981 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg 
     2041 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg 
     2101 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat 
     2161 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc 
     2221 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat 
     2281 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt 
     2341 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag ctttttttaa 
     2401 ggcagttatt ggtgccctta aacgcctggt tgctacgcct gaataagtga taataagcgg 
     2461 atgaatggca gaaattcgaa agcaaattcg acccggtcgt cggttcaggg cagggtcgtt 
     2521 aaatagccgc ttatgtctat tgctggtctc ggtacccggg ttaatgcgcc gctacagggc 
     2581 gcgtccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt 
     2641 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc 
     2701 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac 
     2761 tatagggcga attgggccct ctagatgcat gctcgagcgg ccgccagtgt gatggatatc 
     2821 tgcagaattc gcccttaagg gcgaattcca gcacactggc ggccgttact agtggatccg 
     2881 agctcggtac caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat 
     2941 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc 
     3001 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga 
     3061 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt 
     3121 attgggcgct agatcccatc acatatacct gccgttcact attatttagt gaaatgagat 
     3181 attatgatat tttctgaatt gtgattaaaa aggcaacttt atgcccatgc aacagaaact 
     3241 ataaaaaata cagagaatga aaagaaacag atagattttt tagttcttta ggcccgtagt 
     3301 ctgcaaatcc ttttatgatt ttctatcaaa caaaagagga aaatagacca gttgcaatcc 
     3361 aaacgagagt ctaatagaat gaggtcgaaa agtaaatcgc gcgggtttgt tactgataaa 
     3421 gcaggcaaga cctaaaatgt gtaaagggca aagtgtatac tttggcgtca ccccttacat 
     3481 attttaggtc tttttttatt gtgcgtaact aacttgccat cttcaaacag gagggctgga 
     3541 agaagcagac cgctaacaca gtacataaaa aaggagacat gaacgatgaa catcaaaaag 
     3601 tttgcaaaac aagcaacagt attaaccttt actaccgcac tgctggcagg aggcgcaact 
     3661 caagcgtttg cgaaagaaac gaaccaaaag ccatataagg aaacatacgg catttcccat 
     3721 attacacgcc atgatatgct gcaaatccct gaacagcaaa aaaatgaaaa atatcaagtt 
     3781 cctgagttcg attcgtccac aattaaaaat atctcttctg caaaaggcct ggacgtttgg 
     3841 gacagctggc cattacaaaa cgctgacggc actgtcgcaa actatcacgg ctaccacatc 
     3901 gtctttgcat tagccggaga tcctaaaaat gcggatgaca catcgattta catgttctat 
     3961 caaaaagtcg gcgaaacttc tattgacagc tggaaaaacg ctggccgcgt ctttaaagac 
     4021 agcgacaaat tcgatgcaaa tgattctatc ctaaaagacc aaacacaaga atggtcaggt 
     4081 tcagccacat ttacatctga cggaaaaatc cgtttattct acactgattt ctccggtaaa 
     4141 cattacggca aacaaacact gacaactgca caagttaacg tatcagcatc agacagctct 
     4201 ttgaacatca acggtgtaga ggattataaa tcaatctttg acggtgacgg aaaaacgtat 
     4261 caaaatgtac agcagttcat cgatgaaggc aactacagct caggcgacaa ccatacgctg 
     4321 agagatcctc actacgtaga agataaaggc cacaaatact tagtatttga agcaaacact 
     4381 ggaactgaag atggctacca aggcgaagaa tctttattta acaaagcata ctatggcaaa 
     4441 agcacatcat tcttccgtca agaaagtcaa aaacttctgc aaagcgataa aaaacgcacg 
     4501 gctgagttag caaacggcgc tctcggtatg attgagctaa acgatgatta cacactgaaa 
     4561 aaagtgatga aaccgctgat tgcatctaac acagtaacag atgaaattga acgcgcgaac 
     4621 gtctttaaaa tgaacggcaa atggtacctg ttcactgact cccgcggatc aaaaatgacg 
     4681 attgacggca ttacgtctaa cgatatttac atgcttggtt atgtttctaa ttctttaact 
     4741 ggcccataca agccgctgaa caaaactggc cttgtgttaa aaatggatct tgatcctaac 
     4801 gatgtaacct ttacttactc acacttcgct gtacctcaag cgaaaggaaa caatgtcgtg 
     4861 attacaagct atatgacaaa cagaggattc tacgcagaca aacaatcaac gtttgcgcca 
     4921 agcttcctgc tgaacatcaa aggcaagaaa acatctgttg tcaaagacag catccttgaa 
     4981 caaggacaat taacagttaa caaataaaaa cgcaaaagaa aatgccgata ttgactaccg 
     5041 gaagcagtgt gaccgtgtgc ttctcaaatg cctgattcag gctgtctatg tgtgactgtt 
     5101 gagctgtaac aagttgtctc aggtgttcaa tttcatgttc tagttgcttt gttttactgg 
     5161 tttcacctgt tctattaggt gttacatgct gttcatctgt tacattgtcg atctgttcat 
     5221 ggtgaacagc tttaaatgca ccaaaaactc gtaaaagctc tgatgtatct atctttttta 
     5281 caccgttttc atctgtgcat atggacagtt ttccctttga tatgtaacgg tgaacagttg 
     5341 ttctactttt gtttgttagt cttgatgctt cactgataga tacaagagcc ataagaacct 
     5401 cagagcgctt ggagccaccc gcagttcgaa aaataaggat cccacgtcac tattgtatac 
     5461 tctatattat actctatgtt atactctgta atcctactca ataaacgtgt cacgcctgtg 
     5521 aaaccgtact aagtctcccg tgtcttctta tcaccatcag gtgacatcct cgcccaggct 
     5581 gtcaatcatg ccggtatcga ttccagtagc accggcccca cgctgacaac ccactcttgc 
     5641 agcgttagca gcgcccctct taacaagccg acccccacca gcgtcgcggt tactaacact 
     5701 cctctccccg acctgcagcc caagctt 
//
