LOCUS       pAL1254\for\webs        5856 bp    DNA     linear       12-SEP-2008
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|493233508|
COMMENT     VNTDBDATE|493233653|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAL1254 for website|
COMMENT     VNTAUTHORNAME|Rich Stanton|
FEATURES             Location/Qualifiers
     misc_feature    308..326
                     /vntifkey="21"
                     /label=tet\operator
     misc_feature    329..347
                     /vntifkey="21"
                     /label=tet\Operator
     promoter        21..430
                     /vntifkey="29"
                     /label=CMV\Promotor
     misc_feature    486..491
                     /vntifkey="21"
                     /label=Link
     CDS             444..485
                     /vntifkey="4"
                     /label=V5\Tag
     promoter        600..698
                     /invitrogen="2330000"
                     /vntifkey="30"
                     /label=bla\promoter
     CDS             699..1559
                     /invitrogen="1020000"
                     /vntifkey="4"
                     /label=Amp(R)
     primer          492..513
                     /vntifkey="27"
                     /label=Primer\-L
     CDS             2891..4312
                     /vntifkey="4"
                     /label=sacB
     CDS             complement(1866..2219)
                     /vntifkey="4"
                     /label=LacZa
     primer          complement(5544..5564)
                     /vntifkey="27"
                     /label=Primer-R
     polyA_signal    5571..5849
                     /vntifkey="25"
                     /label=CMV\IE\PolyA
BASE COUNT     1671 a      1394 c      1364 g      1427 t 
ORIGIN
        1 aagcttgcat gcctgcaggt ctggcattat gcccagtaca tgaccttatg ggactttcct 
       61 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag 
      121 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt 
      181 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac 
      241 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc 
      301 agagctctcc ctatcagtga tagagatctc cctatcagtg atagagagtt tagtgaaccg 
      361 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg 
      421 atccagcctg gatccccacc atgggtaagc caatccctaa cccgctccta ggtcttgatt 
      481 ctacgggcgc ccctgtgacg gaagatcact tcgattgtac tgagagtcga ccatagggga 
      541 tcgggagatc tcccgatccg tcgacgtcag gtggcacttt tcggggaaat gtgcgcggaa 
      601 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac 
      661 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg 
      721 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc 
      781 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg 
      841 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga 
      901 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc 
      961 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag 
     1021 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga 
     1081 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg 
     1141 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga 
     1201 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt 
     1261 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact 
     1321 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt 
     1381 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg 
     1441 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta 
     1501 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac 
     1561 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta 
     1621 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt 
     1681 tttcgttcca ctgagctttt tttaaggcag ttattggtgc ccttaaacgc ctggttgcta 
     1741 cgcctgaata agtgataata agcggatgaa tggcagaaat tcgaaagcaa attcgacccg 
     1801 gtcgtcggtt cagggcaggg tcgttaaata gccgcttatg tctattgctg gtctcggtac 
     1861 ccgggttaat gcgccgctac agggcgcgtc cattcgccat tcaggctgcg caactgttgg 
     1921 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct 
     1981 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg 
     2041 gccagtgaat tgtaatacga ctcactatag ggcgaattgg gccctctaga tgcatgctcg 
     2101 agcggccgcc agtgtgatgg atatctgcag aattcgccct taagggcgaa ttccagcaca 
     2161 ctggcggccg ttactagtgg atccgagctc ggtaccaagc ttggcgtaat catggtcata 
     2221 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag 
     2281 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 
     2341 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 
     2401 acgcgcgggg agaggcggtt tgcgtattgg gcgctagatc ccatcacata tacctgccgt 
     2461 tcactattat ttagtgaaat gagatattat gatattttct gaattgtgat taaaaaggca 
     2521 actttatgcc catgcaacag aaactataaa aaatacagag aatgaaaaga aacagataga 
     2581 ttttttagtt ctttaggccc gtagtctgca aatcctttta tgattttcta tcaaacaaaa 
     2641 gaggaaaata gaccagttgc aatccaaacg agagtctaat agaatgaggt cgaaaagtaa 
     2701 atcgcgcggg tttgttactg ataaagcagg caagacctaa aatgtgtaaa gggcaaagtg 
     2761 tatactttgg cgtcacccct tacatatttt aggtcttttt ttattgtgcg taactaactt 
     2821 gccatcttca aacaggaggg ctggaagaag cagaccgcta acacagtaca taaaaaagga 
     2881 gacatgaacg atgaacatca aaaagtttgc aaaacaagca acagtattaa cctttactac 
     2941 cgcactgctg gcaggaggcg caactcaagc gtttgcgaaa gaaacgaacc aaaagccata 
     3001 taaggaaaca tacggcattt cccatattac acgccatgat atgctgcaaa tccctgaaca 
     3061 gcaaaaaaat gaaaaatatc aagttcctga gttcgattcg tccacaatta aaaatatctc 
     3121 ttctgcaaaa ggcctggacg tttgggacag ctggccatta caaaacgctg acggcactgt 
     3181 cgcaaactat cacggctacc acatcgtctt tgcattagcc ggagatccta aaaatgcgga 
     3241 tgacacatcg atttacatgt tctatcaaaa agtcggcgaa acttctattg acagctggaa 
     3301 aaacgctggc cgcgtcttta aagacagcga caaattcgat gcaaatgatt ctatcctaaa 
     3361 agaccaaaca caagaatggt caggttcagc cacatttaca tctgacggaa aaatccgttt 
     3421 attctacact gatttctccg gtaaacatta cggcaaacaa acactgacaa ctgcacaagt 
     3481 taacgtatca gcatcagaca gctctttgaa catcaacggt gtagaggatt ataaatcaat 
     3541 ctttgacggt gacggaaaaa cgtatcaaaa tgtacagcag ttcatcgatg aaggcaacta 
     3601 cagctcaggc gacaaccata cgctgagaga tcctcactac gtagaagata aaggccacaa 
     3661 atacttagta tttgaagcaa acactggaac tgaagatggc taccaaggcg aagaatcttt 
     3721 atttaacaaa gcatactatg gcaaaagcac atcattcttc cgtcaagaaa gtcaaaaact 
     3781 tctgcaaagc gataaaaaac gcacggctga gttagcaaac ggcgctctcg gtatgattga 
     3841 gctaaacgat gattacacac tgaaaaaagt gatgaaaccg ctgattgcat ctaacacagt 
     3901 aacagatgaa attgaacgcg cgaacgtctt taaaatgaac ggcaaatggt acctgttcac 
     3961 tgactcccgc ggatcaaaaa tgacgattga cggcattacg tctaacgata tttacatgct 
     4021 tggttatgtt tctaattctt taactggccc atacaagccg ctgaacaaaa ctggccttgt 
     4081 gttaaaaatg gatcttgatc ctaacgatgt aacctttact tactcacact tcgctgtacc 
     4141 tcaagcgaaa ggaaacaatg tcgtgattac aagctatatg acaaacagag gattctacgc 
     4201 agacaaacaa tcaacgtttg cgccaagctt cctgctgaac atcaaaggca agaaaacatc 
     4261 tgttgtcaaa gacagcatcc ttgaacaagg acaattaaca gttaacaaat aacctgttga 
     4321 caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac 
     4381 ccaggaggca gatcatgagt ctgaaagaaa aaacacaatc tctgtttgcc aacgcatttg 
     4441 gctaccctgc cactcacacc attcaggcgc ctggccgcgt gaatttgatt ggtgaacaca 
     4501 ccgactacaa cgacggtttc gttctgccct gcgcgattga ttatcaaacc gtgatcagtt 
     4561 gtgcaccacg cgatgaccgt aaagttcgcg tgatggcagc cgattatgaa aatcagctcg 
     4621 acgagttttc cctcgatgcg cccattgtcg cacatgaaaa ctatcaatgg gctaactacg 
     4681 ttcgtggcgt ggtgaaacat ctgcaactgc gtaacaacag cttcggcggc gtggacatgg 
     4741 tgatcagcgg caatgtgccg cagggtgccg ggttaagttc ttccgcttca ctggaagtcg 
     4801 cggtcggaac cgtattgcag cagctttatc atctgccgct ggacggcgca caaatcgcgc 
     4861 ttaacggtca ggaagcagaa aaccagtttg taggctgtaa ctgcgggatc atggatcagc 
     4921 taatttccgc gctcggcaag aaagatcatg ccttgctgat cgattgccgc tcactgggga 
     4981 ccaaagcagt ttccatgccc aaaggtgtgg ctgtcgtcat catcaacagt aacttcaaac 
     5041 gtaccctggt tggcagcgaa tacaacaccc gtcgtgaaca gtgcgaaacc ggtgcgcgtt 
     5101 tcttccagca gccagccctg cgtgatgtca ccattgaaga gttcaacgct gttgcgcatg 
     5161 aactggaccc gatcgtggca aaacgcgtgc gtcatatact gactgaaaac gcccgcaccg 
     5221 ttgaagctgc cagcgcgctg gagcaaggcg acctgaaacg tatgggcgag ttgatggcgg 
     5281 agtctcatgc ctctatgcgc gatgatttcg aaatcaccgt gccgcaaatt gacactctgg 
     5341 tagaaatcgt caaagctgtg attggcgaca aaggtggcgt acgcatgacc ggcggcggat 
     5401 ttggcggctg tatcgtcgcg ctgatcccgg aagagctggt gcctgccgta cagcaagctg 
     5461 tcgctgaaca atatgaagca aaaacaggta ttaaagagac tttttacgtt tgtaaaccat 
     5521 cacaaggagc aggacagtgc tgacaagagc cataagaacc tcagggatcc cacgtcacta 
     5581 ttgtatactc tatattatac tctatgttat actctgtaat cctactcaat aaacgtgtca 
     5641 cgcctgtgaa accgtactaa gtctcccgtg tcttcttatc accatcaggt gacatcctcg 
     5701 cccaggctgt caatcatgcc ggtatcgatt ccagtagcac cggccccacg ctgacaaccc 
     5761 actcttgcag cgttagcagc gcccctctta acaagccgac ccccaccagc gtcgcggtta 
     5821 ctaacactcc tctccccgac ctgcagccca agcttg 
//
