LOCUS       pAL1239\for\webs        5657 bp    DNA     linear       12-SEP-2008
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|493236732|
COMMENT     VNTDBDATE|493236802|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAL1239 for website|
COMMENT     VNTAUTHORNAME|Rich Stanton|
COMMENT     VNTOAUTHORNAME|Brian|
FEATURES             Location/Qualifiers
     promoter        28..397
                     /vntifkey="29"
                     /label=CMV\Promotor
     promoter        511..609
                     /invitrogen="2330000"
                     /vntifkey="30"
                     /label=bla\promoter
     CDS             610..1470
                     /invitrogen="1020000"
                     /vntifkey="4"
                     /label=Amp(R)
     primer          403..424
                     /vntifkey="27"
                     /label=Primer\-L
     CDS             2802..4223
                     /vntifkey="4"
                     /label=sacB
     primer          complement(4599..4619)
                     /vntifkey="27"
                     /label=Primer-R
     CDS             complement(1777..2130)
                     /vntifkey="4"
                     /label=LacZa
     CDS             4647..5366
                     /vntifkey="4"
                     /label=EGFP
     polyA_signal    5373..5651
                     /vntifkey="25"
                     /label=CMV\IE\PolyA
BASE COUNT     1601 a      1370 c      1304 g      1382 t 
ORIGIN
        1 aagcttgcat gcctgcaggt caattccctg gcattatgcc cagtacatga ccttatggga 
       61 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt 
      121 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca 
      181 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg 
      241 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta 
      301 tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt 
      361 tgacctccat agaagacacc gggaccgatc cagcctggat cccctgtgac ggaagatcac 
      421 ttcgattgta ctgagagtcg accatagggg atcgggagat ctcccgatcc gtcgacgtca 
      481 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat 
      541 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa 
      601 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt 
      661 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag 
      721 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt 
      781 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg 
      841 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag 
      901 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta 
      961 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg 
     1021 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta 
     1081 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac 
     1141 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt 
     1201 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca 
     1261 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag 
     1321 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta 
     1381 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag 
     1441 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt 
     1501 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 
     1561 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcttt ttttaaggca 
     1621 gttattggtg cccttaaacg cctggttgct acgcctgaat aagtgataat aagcggatga 
     1681 atggcagaaa ttcgaaagca aattcgaccc ggtcgtcggt tcagggcagg gtcgttaaat 
     1741 agccgcttat gtctattgct ggtctcggta cccgggttaa tgcgccgcta cagggcgcgt 
     1801 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 
     1861 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 
     1921 gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg actcactata 
     1981 gggcgaattg ggccctctag atgcatgctc gagcggccgc cagtgtgatg gatatctgca 
     2041 gaattcgccc ttaagggcga attccagcac actggcggcc gttactagtg gatccgagct 
     2101 cggtaccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc 
     2161 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat 
     2221 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc 
     2281 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg 
     2341 ggcgctagat cccatcacat atacctgccg ttcactatta tttagtgaaa tgagatatta 
     2401 tgatattttc tgaattgtga ttaaaaaggc aactttatgc ccatgcaaca gaaactataa 
     2461 aaaatacaga gaatgaaaag aaacagatag attttttagt tctttaggcc cgtagtctgc 
     2521 aaatcctttt atgattttct atcaaacaaa agaggaaaat agaccagttg caatccaaac 
     2581 gagagtctaa tagaatgagg tcgaaaagta aatcgcgcgg gtttgttact gataaagcag 
     2641 gcaagaccta aaatgtgtaa agggcaaagt gtatactttg gcgtcacccc ttacatattt 
     2701 taggtctttt tttattgtgc gtaactaact tgccatcttc aaacaggagg gctggaagaa 
     2761 gcagaccgct aacacagtac ataaaaaagg agacatgaac gatgaacatc aaaaagtttg 
     2821 caaaacaagc aacagtatta acctttacta ccgcactgct ggcaggaggc gcaactcaag 
     2881 cgtttgcgaa agaaacgaac caaaagccat ataaggaaac atacggcatt tcccatatta 
     2941 cacgccatga tatgctgcaa atccctgaac agcaaaaaaa tgaaaaatat caagttcctg 
     3001 agttcgattc gtccacaatt aaaaatatct cttctgcaaa aggcctggac gtttgggaca 
     3061 gctggccatt acaaaacgct gacggcactg tcgcaaacta tcacggctac cacatcgtct 
     3121 ttgcattagc cggagatcct aaaaatgcgg atgacacatc gatttacatg ttctatcaaa 
     3181 aagtcggcga aacttctatt gacagctgga aaaacgctgg ccgcgtcttt aaagacagcg 
     3241 acaaattcga tgcaaatgat tctatcctaa aagaccaaac acaagaatgg tcaggttcag 
     3301 ccacatttac atctgacgga aaaatccgtt tattctacac tgatttctcc ggtaaacatt 
     3361 acggcaaaca aacactgaca actgcacaag ttaacgtatc agcatcagac agctctttga 
     3421 acatcaacgg tgtagaggat tataaatcaa tctttgacgg tgacggaaaa acgtatcaaa 
     3481 atgtacagca gttcatcgat gaaggcaact acagctcagg cgacaaccat acgctgagag 
     3541 atcctcacta cgtagaagat aaaggccaca aatacttagt atttgaagca aacactggaa 
     3601 ctgaagatgg ctaccaaggc gaagaatctt tatttaacaa agcatactat ggcaaaagca 
     3661 catcattctt ccgtcaagaa agtcaaaaac ttctgcaaag cgataaaaaa cgcacggctg 
     3721 agttagcaaa cggcgctctc ggtatgattg agctaaacga tgattacaca ctgaaaaaag 
     3781 tgatgaaacc gctgattgca tctaacacag taacagatga aattgaacgc gcgaacgtct 
     3841 ttaaaatgaa cggcaaatgg tacctgttca ctgactcccg cggatcaaaa atgacgattg 
     3901 acggcattac gtctaacgat atttacatgc ttggttatgt ttctaattct ttaactggcc 
     3961 catacaagcc gctgaacaaa actggccttg tgttaaaaat ggatcttgat cctaacgatg 
     4021 taacctttac ttactcacac ttcgctgtac ctcaagcgaa aggaaacaat gtcgtgatta 
     4081 caagctatat gacaaacaga ggattctacg cagacaaaca atcaacgttt gcgccaagct 
     4141 tcctgctgaa catcaaaggc aagaaaacat ctgttgtcaa agacagcatc cttgaacaag 
     4201 gacaattaac agttaacaaa taaaaacgca aaagaaaatg ccgatattga ctaccggaag 
     4261 cagtgtgacc gtgtgcttct caaatgcctg attcaggctg tctatgtgtg actgttgagc 
     4321 tgtaacaagt tgtctcaggt gttcaatttc atgttctagt tgctttgttt tactggtttc 
     4381 acctgttcta ttaggtgtta catgctgttc atctgttaca ttgtcgatct gttcatggtg 
     4441 aacagcttta aatgcaccaa aaactcgtaa aagctctgat gtatctatct tttttacacc 
     4501 gttttcatct gtgcatatgg acagttttcc ctttgatatg taacggtgaa cagttgttct 
     4561 acttttgttt gttagtcttg atgcttcact gatagataca agagccataa gaacctcagg 
     4621 gtagcgctgg atcagcaggg tccgcgatgg tgagcaaggg cgaggagctg ttcaccgggg 
     4681 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg 
     4741 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg 
     4801 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct 
     4861 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag 
     4921 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg 
     4981 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca 
     5041 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct 
     5101 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca 
     5161 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg 
     5221 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc 
     5281 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc 
     5341 tcggcatgga cgagctgtac aagtaaggat cccacgtcac tattgtatac tctatattat 
     5401 actctatgtt atactctgta atcctactca ataaacgtgt cacgcctgtg aaaccgtact 
     5461 aagtctcccg tgtcttctta tcaccatcag gtgacatcct cgcccaggct gtcaatcatg 
     5521 ccggtatcga ttccagtagc accggcccca cgctgacaac ccactcttgc agcgttagca 
     5581 gcgcccctct taacaagccg acccccacca gcgtcgcggt tactaacact cctctccccg 
     5641 acctgcagcc caagctt 
//
