LOCUS       pAL1213\for\webs        5017 bp    DNA     linear       12-SEP-2008
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|493235701|
COMMENT     VNTDBDATE|493235787|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAL1213 for website|
COMMENT     VNTAUTHORNAME|Rich Stanton|
FEATURES             Location/Qualifiers
     CDS             complement(1845..2198)
                     /vntifkey="4"
                     /label=LacZa
     primer          complement(4667..4687)
                     /vntifkey="27"
                     /label=Primer-R
     CDS             2870..4291
                     /vntifkey="4"
                     /label=sacB
     primer          471..492
                     /vntifkey="27"
                     /label=Primer\-L
     CDS             678..1538
                     /invitrogen="1020000"
                     /vntifkey="4"
                     /label=Amp(R)
     promoter        579..677
                     /invitrogen="2330000"
                     /vntifkey="30"
                     /label=bla\promoter
     misc_feature    315..333
                     /vntifkey="21"
                     /label=tet\operator
     misc_feature    336..354
                     /vntifkey="21"
                     /label=tet\Operator
     promoter        21..437
                     /vntifkey="29"
                     /label=CMV\Promotor
     polyA_signal    4739..5017
                     /vntifkey="25"
                     /label=CMV\IE\PolyA
     misc_feature    443..470
                     /vntifkey="21"
                     /label=miR-155\For
     misc_feature    4688..4732
                     /vntifkey="21"
                     /label=miR-155\Rev
BASE COUNT     1452 a      1149 c      1117 g      1299 t 
ORIGIN
        1 aagcttgcat gcctgcaggt caattccctg gcattatgcc cagtacatga ccttatggga 
       61 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt 
      121 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca 
      181 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg 
      241 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta 
      301 tataagcaga gctctcccta tcagtgatag agatctccct atcagtgata gagagtttag 
      361 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc 
      421 gggaccgatc cagcctggat ccctggaggc ttgctgaagg ctgtatgctg cctgtgacgg 
      481 aagatcactt cgattgtact gagagtcgac cataggggat cgggagatct cccgatccgt 
      541 cgacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct 
      601 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat 
      661 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg 
      721 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg 
      781 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc 
      841 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat 
      901 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact 
      961 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca 
     1021 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact 
     1081 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg 
     1141 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg 
     1201 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg 
     1261 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg 
     1321 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag 
     1381 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc 
     1441 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga 
     1501 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat 
     1561 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc 
     1621 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcttttt 
     1681 ttaaggcagt tattggtgcc cttaaacgcc tggttgctac gcctgaataa gtgataataa 
     1741 gcggatgaat ggcagaaatt cgaaagcaaa ttcgacccgg tcgtcggttc agggcagggt 
     1801 cgttaaatag ccgcttatgt ctattgctgg tctcggtacc cgggttaatg cgccgctaca 
     1861 gggcgcgtcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc 
     1921 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta 
     1981 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac 
     2041 tcactatagg gcgaattggg ccctctagat gcatgctcga gcggccgcca gtgtgatgga 
     2101 tatctgcaga attcgccctt aagggcgaat tccagcacac tggcggccgt tactagtgga 
     2161 tccgagctcg gtaccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg 
     2221 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 
     2281 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 
     2341 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 
     2401 gcgtattggg cgctagatcc catcacatat acctgccgtt cactattatt tagtgaaatg 
     2461 agatattatg atattttctg aattgtgatt aaaaaggcaa ctttatgccc atgcaacaga 
     2521 aactataaaa aatacagaga atgaaaagaa acagatagat tttttagttc tttaggcccg 
     2581 tagtctgcaa atccttttat gattttctat caaacaaaag aggaaaatag accagttgca 
     2641 atccaaacga gagtctaata gaatgaggtc gaaaagtaaa tcgcgcgggt ttgttactga 
     2701 taaagcaggc aagacctaaa atgtgtaaag ggcaaagtgt atactttggc gtcacccctt 
     2761 acatatttta ggtctttttt tattgtgcgt aactaacttg ccatcttcaa acaggagggc 
     2821 tggaagaagc agaccgctaa cacagtacat aaaaaaggag acatgaacga tgaacatcaa 
     2881 aaagtttgca aaacaagcaa cagtattaac ctttactacc gcactgctgg caggaggcgc 
     2941 aactcaagcg tttgcgaaag aaacgaacca aaagccatat aaggaaacat acggcatttc 
     3001 ccatattaca cgccatgata tgctgcaaat ccctgaacag caaaaaaatg aaaaatatca 
     3061 agttcctgag ttcgattcgt ccacaattaa aaatatctct tctgcaaaag gcctggacgt 
     3121 ttgggacagc tggccattac aaaacgctga cggcactgtc gcaaactatc acggctacca 
     3181 catcgtcttt gcattagccg gagatcctaa aaatgcggat gacacatcga tttacatgtt 
     3241 ctatcaaaaa gtcggcgaaa cttctattga cagctggaaa aacgctggcc gcgtctttaa 
     3301 agacagcgac aaattcgatg caaatgattc tatcctaaaa gaccaaacac aagaatggtc 
     3361 aggttcagcc acatttacat ctgacggaaa aatccgttta ttctacactg atttctccgg 
     3421 taaacattac ggcaaacaaa cactgacaac tgcacaagtt aacgtatcag catcagacag 
     3481 ctctttgaac atcaacggtg tagaggatta taaatcaatc tttgacggtg acggaaaaac 
     3541 gtatcaaaat gtacagcagt tcatcgatga aggcaactac agctcaggcg acaaccatac 
     3601 gctgagagat cctcactacg tagaagataa aggccacaaa tacttagtat ttgaagcaaa 
     3661 cactggaact gaagatggct accaaggcga agaatcttta tttaacaaag catactatgg 
     3721 caaaagcaca tcattcttcc gtcaagaaag tcaaaaactt ctgcaaagcg ataaaaaacg 
     3781 cacggctgag ttagcaaacg gcgctctcgg tatgattgag ctaaacgatg attacacact 
     3841 gaaaaaagtg atgaaaccgc tgattgcatc taacacagta acagatgaaa ttgaacgcgc 
     3901 gaacgtcttt aaaatgaacg gcaaatggta cctgttcact gactcccgcg gatcaaaaat 
     3961 gacgattgac ggcattacgt ctaacgatat ttacatgctt ggttatgttt ctaattcttt 
     4021 aactggccca tacaagccgc tgaacaaaac tggccttgtg ttaaaaatgg atcttgatcc 
     4081 taacgatgta acctttactt actcacactt cgctgtacct caagcgaaag gaaacaatgt 
     4141 cgtgattaca agctatatga caaacagagg attctacgca gacaaacaat caacgtttgc 
     4201 gccaagcttc ctgctgaaca tcaaaggcaa gaaaacatct gttgtcaaag acagcatcct 
     4261 tgaacaagga caattaacag ttaacaaata aaaacgcaaa agaaaatgcc gatattgact 
     4321 accggaagca gtgtgaccgt gtgcttctca aatgcctgat tcaggctgtc tatgtgtgac 
     4381 tgttgagctg taacaagttg tctcaggtgt tcaatttcat gttctagttg ctttgtttta 
     4441 ctggtttcac ctgttctatt aggtgttaca tgctgttcat ctgttacatt gtcgatctgt 
     4501 tcatggtgaa cagctttaaa tgcaccaaaa actcgtaaaa gctctgatgt atctatcttt 
     4561 tttacaccgt tttcatctgt gcatatggac agttttccct ttgatatgta acggtgaaca 
     4621 gttgttctac ttttgtttgt tagtcttgat gcttcactga tagatacaag agccataaga 
     4681 acctcagcag gacacaaggc ctgttactag cactcacatg gaacaaatgg ccggatccca 
     4741 cgtcactatt gtatactcta tattatactc tatgttatac tctgtaatcc tactcaataa 
     4801 acgtgtcacg cctgtgaaac cgtactaagt ctcccgtgtc ttcttatcac catcaggtga 
     4861 catcctcgcc caggctgtca atcatgccgg tatcgattcc agtagcaccg gccccacgct 
     4921 gacaacccac tcttgcagcg ttagcagcgc ccctcttaac aagccgaccc ccaccagcgt 
     4981 cgcggttact aacactcctc tccccgacct gcagccc 
//
