LOCUS       pAL1212\for\webs        5682 bp    DNA     linear       12-SEP-2008
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|493235163|
COMMENT     VNTDBDATE|493235392|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAL1212 for website|
COMMENT     VNTAUTHORNAME|Rich Stanton|
FEATURES             Location/Qualifiers
     CDS             complement(1817..2170)
                     /vntifkey="4"
                     /label=LacZa
     primer          complement(4639..4659)
                     /vntifkey="27"
                     /label=Primer-R
     CDS             2842..4263
                     /vntifkey="4"
                     /label=sacB
     primer          443..464
                     /vntifkey="27"
                     /label=Primer\-L
     CDS             650..1510
                     /invitrogen="1020000"
                     /vntifkey="4"
                     /label=Amp(R)
     promoter        551..649
                     /invitrogen="2330000"
                     /vntifkey="30"
                     /label=bla\promoter
     misc_feature    315..333
                     /vntifkey="21"
                     /label=tet\operator
     misc_feature    336..354
                     /vntifkey="21"
                     /label=tet\Operator
     promoter        21..437
                     /vntifkey="29"
                     /label=CMV\Promotor
     polyA_signal    5404..5682
                     /vntifkey="25"
                     /label=CMV\IE\PolyA
     misc_feature    4687..5397
                     /vntifkey="21"
                     /label=mCherry
     misc_feature    4660..4686
                     /vntifkey="21"
                     /label=linker
BASE COUNT     1601 a      1363 c      1329 g      1389 t 
ORIGIN
        1 aagcttgcat gcctgcaggt caattccctg gcattatgcc cagtacatga ccttatggga 
       61 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt 
      121 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca 
      181 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg 
      241 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta 
      301 tataagcaga gctctcccta tcagtgatag agatctccct atcagtgata gagagtttag 
      361 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc 
      421 gggaccgatc cagcctggat cccctgtgac ggaagatcac ttcgattgta ctgagagtcg 
      481 accatagggg atcgggagat ctcccgatcc gtcgacgtca ggtggcactt ttcggggaaa 
      541 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat 
      601 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca 
      661 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca 
      721 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta 
      781 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt 
      841 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc 
      901 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc 
      961 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc 
     1021 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa 
     1081 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga 
     1141 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat 
     1201 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca 
     1261 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc 
     1321 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat 
     1381 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag 
     1441 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa 
     1501 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca 
     1561 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc 
     1621 ttaacgtgag ttttcgttcc actgagcttt ttttaaggca gttattggtg cccttaaacg 
     1681 cctggttgct acgcctgaat aagtgataat aagcggatga atggcagaaa ttcgaaagca 
     1741 aattcgaccc ggtcgtcggt tcagggcagg gtcgttaaat agccgcttat gtctattgct 
     1801 ggtctcggta cccgggttaa tgcgccgcta cagggcgcgt ccattcgcca ttcaggctgc 
     1861 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag 
     1921 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt 
     1981 gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattg ggccctctag 
     2041 atgcatgctc gagcggccgc cagtgtgatg gatatctgca gaattcgccc ttaagggcga 
     2101 attccagcac actggcggcc gttactagtg gatccgagct cggtaccaag cttggcgtaa 
     2161 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata 
     2221 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta 
     2281 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa 
     2341 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctagat cccatcacat 
     2401 atacctgccg ttcactatta tttagtgaaa tgagatatta tgatattttc tgaattgtga 
     2461 ttaaaaaggc aactttatgc ccatgcaaca gaaactataa aaaatacaga gaatgaaaag 
     2521 aaacagatag attttttagt tctttaggcc cgtagtctgc aaatcctttt atgattttct 
     2581 atcaaacaaa agaggaaaat agaccagttg caatccaaac gagagtctaa tagaatgagg 
     2641 tcgaaaagta aatcgcgcgg gtttgttact gataaagcag gcaagaccta aaatgtgtaa 
     2701 agggcaaagt gtatactttg gcgtcacccc ttacatattt taggtctttt tttattgtgc 
     2761 gtaactaact tgccatcttc aaacaggagg gctggaagaa gcagaccgct aacacagtac 
     2821 ataaaaaagg agacatgaac gatgaacatc aaaaagtttg caaaacaagc aacagtatta 
     2881 acctttacta ccgcactgct ggcaggaggc gcaactcaag cgtttgcgaa agaaacgaac 
     2941 caaaagccat ataaggaaac atacggcatt tcccatatta cacgccatga tatgctgcaa 
     3001 atccctgaac agcaaaaaaa tgaaaaatat caagttcctg agttcgattc gtccacaatt 
     3061 aaaaatatct cttctgcaaa aggcctggac gtttgggaca gctggccatt acaaaacgct 
     3121 gacggcactg tcgcaaacta tcacggctac cacatcgtct ttgcattagc cggagatcct 
     3181 aaaaatgcgg atgacacatc gatttacatg ttctatcaaa aagtcggcga aacttctatt 
     3241 gacagctgga aaaacgctgg ccgcgtcttt aaagacagcg acaaattcga tgcaaatgat 
     3301 tctatcctaa aagaccaaac acaagaatgg tcaggttcag ccacatttac atctgacgga 
     3361 aaaatccgtt tattctacac tgatttctcc ggtaaacatt acggcaaaca aacactgaca 
     3421 actgcacaag ttaacgtatc agcatcagac agctctttga acatcaacgg tgtagaggat 
     3481 tataaatcaa tctttgacgg tgacggaaaa acgtatcaaa atgtacagca gttcatcgat 
     3541 gaaggcaact acagctcagg cgacaaccat acgctgagag atcctcacta cgtagaagat 
     3601 aaaggccaca aatacttagt atttgaagca aacactggaa ctgaagatgg ctaccaaggc 
     3661 gaagaatctt tatttaacaa agcatactat ggcaaaagca catcattctt ccgtcaagaa 
     3721 agtcaaaaac ttctgcaaag cgataaaaaa cgcacggctg agttagcaaa cggcgctctc 
     3781 ggtatgattg agctaaacga tgattacaca ctgaaaaaag tgatgaaacc gctgattgca 
     3841 tctaacacag taacagatga aattgaacgc gcgaacgtct ttaaaatgaa cggcaaatgg 
     3901 tacctgttca ctgactcccg cggatcaaaa atgacgattg acggcattac gtctaacgat 
     3961 atttacatgc ttggttatgt ttctaattct ttaactggcc catacaagcc gctgaacaaa 
     4021 actggccttg tgttaaaaat ggatcttgat cctaacgatg taacctttac ttactcacac 
     4081 ttcgctgtac ctcaagcgaa aggaaacaat gtcgtgatta caagctatat gacaaacaga 
     4141 ggattctacg cagacaaaca atcaacgttt gcgccaagct tcctgctgaa catcaaaggc 
     4201 aagaaaacat ctgttgtcaa agacagcatc cttgaacaag gacaattaac agttaacaaa 
     4261 taaaaacgca aaagaaaatg ccgatattga ctaccggaag cagtgtgacc gtgtgcttct 
     4321 caaatgcctg attcaggctg tctatgtgtg actgttgagc tgtaacaagt tgtctcaggt 
     4381 gttcaatttc atgttctagt tgctttgttt tactggtttc acctgttcta ttaggtgtta 
     4441 catgctgttc atctgttaca ttgtcgatct gttcatggtg aacagcttta aatgcaccaa 
     4501 aaactcgtaa aagctctgat gtatctatct tttttacacc gttttcatct gtgcatatgg 
     4561 acagttttcc ctttgatatg taacggtgaa cagttgttct acttttgttt gttagtcttg 
     4621 atgcttcact gatagataca agagccataa gaacctcagg gtagcgctgg atcagcaggg 
     4681 tccgcgatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc 
     4741 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc 
     4801 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc 
     4861 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg 
     4921 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg 
     4981 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg 
     5041 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc 
     5101 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag 
     5161 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac 
     5221 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac 
     5281 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag 
     5341 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtaggga 
     5401 tcccacgtca ctattgtata ctctatatta tactctatgt tatactctgt aatcctactc 
     5461 aataaacgtg tcacgcctgt gaaaccgtac taagtctccc gtgtcttctt atcaccatca 
     5521 ggtgacatcc tcgcccaggc tgtcaatcat gccggtatcg attccagtag caccggcccc 
     5581 acgctgacaa cccactcttg cagcgttagc agcgcccctc ttaacaagcc gacccccacc 
     5641 agcgtcgcgg ttactaacac tcctctcccc gacctgcagc cc 
//
