LOCUS       pAL1141\cassette        4991 bp    DNA     linear       12-DEC-2008
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|501520719|
COMMENT     VNTDBDATE|501520719|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAL1141 cassette only for website|
COMMENT     VNTAUTHORNAME|Rich Stanton|
FEATURES             Location/Qualifiers
     polyA_signal    4707..4985
                     /vntifkey="25"
                     /label=CMV\IE\PolyA
     promoter        11..427
                     /vntifkey="29"
                     /label=CMV\Promotor
     misc_feature    326..344
                     /vntifkey="21"
                     /label=tet\Operator
     misc_feature    305..323
                     /vntifkey="21"
                     /label=tet\operator
     CDS             complement(1807..2160)
                     /vntifkey="4"
                     /label=LacZa
     primer          complement(4629..4649)
                     /vntifkey="27"
                     /label=Primer-R
     CDS             2832..4253
                     /vntifkey="4"
                     /label=sacB
     primer          433..454
                     /vntifkey="27"
                     /label=Primer\-L
     CDS             640..1500
                     /invitrogen="1020000"
                     /vntifkey="4"
                     /label=Amp(R)
     promoter        541..639
                     /invitrogen="2330000"
                     /vntifkey="30"
                     /label=bla\promoter
     misc_feature    4656..4700
                     /vntifkey="21"
                     /label=V5\tag
     misc_feature    4650..4655
                     /vntifkey="21"
                     /label=link
BASE COUNT     1444 a      1146 c      1105 g      1296 t 
ORIGIN
        1 gcctgcaggt caattccctg gcattatgcc cagtacatga ccttatggga ctttcctact 
       61 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac 
      121 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac 
      181 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac 
      241 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga 
      301 gctctcccta tcagtgatag agatctccct atcagtgata gagagtttag tgaaccgtca 
      361 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc 
      421 cagcctggat cccctgtgac ggaagatcac ttcgattgta ctgagagtcg accatagggg 
      481 atcgggagat ctcccgatcc gtcgacgtca ggtggcactt ttcggggaaa tgtgcgcgga 
      541 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa 
      601 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt 
      661 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg 
      721 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg 
      781 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg 
      841 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag 
      901 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca 
      961 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg 
     1021 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc 
     1081 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg 
     1141 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg 
     1201 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac 
     1261 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg 
     1321 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg 
     1381 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact 
     1441 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa 
     1501 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt 
     1561 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag 
     1621 ttttcgttcc actgagcttt ttttaaggca gttattggtg cccttaaacg cctggttgct 
     1681 acgcctgaat aagtgataat aagcggatga atggcagaaa ttcgaaagca aattcgaccc 
     1741 ggtcgtcggt tcagggcagg gtcgttaaat agccgcttat gtctattgct ggtctcggta 
     1801 cccgggttaa tgcgccgcta cagggcgcgt ccattcgcca ttcaggctgc gcaactgttg 
     1861 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc 
     1921 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac 
     1981 ggccagtgaa ttgtaatacg actcactata gggcgaattg ggccctctag atgcatgctc 
     2041 gagcggccgc cagtgtgatg gatatctgca gaattcgccc ttaagggcga attccagcac 
     2101 actggcggcc gttactagtg gatccgagct cggtaccaag cttggcgtaa tcatggtcat 
     2161 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa 
     2221 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc 
     2281 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc 
     2341 aacgcgcggg gagaggcggt ttgcgtattg ggcgctagat cccatcacat atacctgccg 
     2401 ttcactatta tttagtgaaa tgagatatta tgatattttc tgaattgtga ttaaaaaggc 
     2461 aactttatgc ccatgcaaca gaaactataa aaaatacaga gaatgaaaag aaacagatag 
     2521 attttttagt tctttaggcc cgtagtctgc aaatcctttt atgattttct atcaaacaaa 
     2581 agaggaaaat agaccagttg caatccaaac gagagtctaa tagaatgagg tcgaaaagta 
     2641 aatcgcgcgg gtttgttact gataaagcag gcaagaccta aaatgtgtaa agggcaaagt 
     2701 gtatactttg gcgtcacccc ttacatattt taggtctttt tttattgtgc gtaactaact 
     2761 tgccatcttc aaacaggagg gctggaagaa gcagaccgct aacacagtac ataaaaaagg 
     2821 agacatgaac gatgaacatc aaaaagtttg caaaacaagc aacagtatta acctttacta 
     2881 ccgcactgct ggcaggaggc gcaactcaag cgtttgcgaa agaaacgaac caaaagccat 
     2941 ataaggaaac atacggcatt tcccatatta cacgccatga tatgctgcaa atccctgaac 
     3001 agcaaaaaaa tgaaaaatat caagttcctg agttcgattc gtccacaatt aaaaatatct 
     3061 cttctgcaaa aggcctggac gtttgggaca gctggccatt acaaaacgct gacggcactg 
     3121 tcgcaaacta tcacggctac cacatcgtct ttgcattagc cggagatcct aaaaatgcgg 
     3181 atgacacatc gatttacatg ttctatcaaa aagtcggcga aacttctatt gacagctgga 
     3241 aaaacgctgg ccgcgtcttt aaagacagcg acaaattcga tgcaaatgat tctatcctaa 
     3301 aagaccaaac acaagaatgg tcaggttcag ccacatttac atctgacgga aaaatccgtt 
     3361 tattctacac tgatttctcc ggtaaacatt acggcaaaca aacactgaca actgcacaag 
     3421 ttaacgtatc agcatcagac agctctttga acatcaacgg tgtagaggat tataaatcaa 
     3481 tctttgacgg tgacggaaaa acgtatcaaa atgtacagca gttcatcgat gaaggcaact 
     3541 acagctcagg cgacaaccat acgctgagag atcctcacta cgtagaagat aaaggccaca 
     3601 aatacttagt atttgaagca aacactggaa ctgaagatgg ctaccaaggc gaagaatctt 
     3661 tatttaacaa agcatactat ggcaaaagca catcattctt ccgtcaagaa agtcaaaaac 
     3721 ttctgcaaag cgataaaaaa cgcacggctg agttagcaaa cggcgctctc ggtatgattg 
     3781 agctaaacga tgattacaca ctgaaaaaag tgatgaaacc gctgattgca tctaacacag 
     3841 taacagatga aattgaacgc gcgaacgtct ttaaaatgaa cggcaaatgg tacctgttca 
     3901 ctgactcccg cggatcaaaa atgacgattg acggcattac gtctaacgat atttacatgc 
     3961 ttggttatgt ttctaattct ttaactggcc catacaagcc gctgaacaaa actggccttg 
     4021 tgttaaaaat ggatcttgat cctaacgatg taacctttac ttactcacac ttcgctgtac 
     4081 ctcaagcgaa aggaaacaat gtcgtgatta caagctatat gacaaacaga ggattctacg 
     4141 cagacaaaca atcaacgttt gcgccaagct tcctgctgaa catcaaaggc aagaaaacat 
     4201 ctgttgtcaa agacagcatc cttgaacaag gacaattaac agttaacaaa taaaaacgca 
     4261 aaagaaaatg ccgatattga ctaccggaag cagtgtgacc gtgtgcttct caaatgcctg 
     4321 attcaggctg tctatgtgtg actgttgagc tgtaacaagt tgtctcaggt gttcaatttc 
     4381 atgttctagt tgctttgttt tactggtttc acctgttcta ttaggtgtta catgctgttc 
     4441 atctgttaca ttgtcgatct gttcatggtg aacagcttta aatgcaccaa aaactcgtaa 
     4501 aagctctgat gtatctatct tttttacacc gttttcatct gtgcatatgg acagttttcc 
     4561 ctttgatatg taacggtgaa cagttgttct acttttgttt gttagtcttg atgcttcact 
     4621 gatagataca agagccataa gaacctcaga gcgctggtaa gccaatccct aacccgctcc 
     4681 taggtcttga ttctacgtaa ggatcccacg tcactattgt atactctata ttatactcta 
     4741 tgttatactc tgtaatccta ctcaataaac gtgtcacgcc tgtgaaaccg tactaagtct 
     4801 cccgtgtctt cttatcacca tcaggtgaca tcctcgccca ggctgtcaat catgccggta 
     4861 tcgattccag tagcaccggc cccacgctga caacccactc ttgcagcgtt agcagcgccc 
     4921 ctcttaacaa gccgaccccc accagcgtcg cggttactaa cactcctctc cccgacctgc 
     4981 agcccaagct t 
//
